Thymosin β4 released by mast cells under stress conditions impairs intestinal epithelial barrier via IL22RA1/JAK1/STAT3 signaling in irritable bowel syndrome.
This study investigated the role of mast cell (MC)-derived thymosin β4 (Tβ4) in stress-induced intestinal barrier dysfunction in irritable bowel syndrome (IBS). Researchers measured Tβ4 levels in colonic mucus of IBS patients and used a combination of in vitro experiments, rodent models (including Tβ4-knockout rats and MC-deficient mice), and reconstitution experiments to examine mechanisms. The study found that Tβ4 levels were elevated in the colonic mucosa and intestinal MCs of IBS patients. In animal models, Tβ4 appeared to reduce tight junction proteins and the IL22RA1/Reg3γ cascade while increasing myosin light chain kinase activity, collectively impairing the epithelial barrier. Tβ4-deficient rats showed resistance to stress-induced barrier disruption, and reintroduction of Tβ4 or wild-type peritoneal MCs restored that disruption. Mechanistically, Tβ4 release from MCs was found to depend on corticotropin-releasing hormone receptor 1 signaling rather than classic degranulation, and its barrier-impairing effects were linked to inhibition of the IL22RA1/JAK1/STAT3 signaling pathway. Limitations include reliance on rodent models for mechanistic work, modest human clinical data, and the complexity of translating these findings to therapeutic applications.
Why this grade: While the study includes human mucosal Tβ4 measurements in IBS patients, the mechanistic and causal evidence is derived predominantly from rodent genetic knockout and reconstitution models, placing overall evidence in the preclinical-mixed category.
Background Mast cells (MCs) under stress conditions contribute to the development of irritable bowel syndrome (IBS), yet their precise mechanisms in IBS remain unclear. Aim To investigate the role of MC-derived thymosin β4 (Tβ4) in stress-induced intestinal barrier dysfunction. Methods The colonic mucus Tβ4 levels in IBS patients were determined and their effects on the epithelial barrier were assessed in vitro and in vivo . Specifically, rats genetically deficient in Tβ4 ( Tβ4 -/- ) or mice deficient in MCs ( Kit w-sh/w-sh ) were used to observe the effects of reintroducing Tβ4 or wild-type peritoneal MCs (wt-PMCs) into these animals. Additionally, the regulatory mechanism underlying Tβ4 secretion in MCs was investigated. Results We demonstrated that high levels of Tβ4 in IBS mucus and intestinal MCs mediate stress-associated disruptive changes to the epithelial barrier. Moreover, Tβ4 treatment of wild-type or MC-deficient Kit w-sh/w-sh mice caused a reduction in tight junction proteins and the interleukin 22 receptor A1 (IL22RA1)/Reg3γ cascade, but an increase in myosin light chain kinase. Furthermore, Tβ4 -/- rats were resistant to stress, though reintroduction of Tβ4 or wt-PMCs restored stress or corticotropin-releasing hormone (CRH)-induced barrier disturbance. Consistently, Tβ4 release from MCs was dependent on the CRH receptor 1, but not degranulation. The effect of Tβ4 was accompanied by IL22RA1/Janus kinase 1 (JAK1)/signal transducer and activation of transcription 3 (STAT3) pathway inhibition, suggesting a mechanism for physical and immune barrier suppression. Conclusion Collectively, these results suggest that Tβ4, which is abundant in IBS mucus and the secretome of MCs, plays a crucial role in the pathogenesis of IBS via IL22RA1/JAK1/STAT3 signaling, with potential implications for diagnostic and therapeutic targeting.
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